Figure 5: Involvement of calpain and caspase-12 in apoptosis. (A) In situ immunofluorescent labeling detected an increase in calpain expression and also cell death with calpain overexpression, suggesting calpain involvement in apoptosis. (B) In situ immunofluorescent labeling showed caspase-12 overexpression and also cell death with caspase-12 overexpression following ATRA and IFN-γ combination therapy. In the bar diagrams, x-axis denotes 1 = CTL, 2 = ATRA, 3 = IFN-γ, and 4 = ATRA plus IFN-γ.