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Figure 4: Cytotoxicity of effector CTL against HMEC. Target HMEC cells (3.7 × 104 cells/well) were incubated with effector CTL at a ratio of 1:8. After 3 days, floating CTL were removed, target cells were carefully washed with HBSS, and attached HMEC were trypsinized and cell viability determined. Bars correspond to images in Figure 4 according to literal notation. The number of calculated HMEC present in control cultures was set as 100%. ** - p < 0.01; * - p < 0.05, additional marks indicate bars paired to calculate p. Data represent the mean value of 3 identical measurements ± SD. |