Figure 1: A549 lung cancer cells were treated with 100 ÁM of linoleic acid (LA), docosahexaenoic acid (DHA), or ethanol (EtOH), or 25 nM of control/nonsilencing siRNA (NS siRNA), or siRNA specific to p53 (p53 siRNA). 24 hours later siRNA treatments were supplemented with 100 ÁM of LA, DHA, or EtOH. 48 hours after PUFA supplementation, cells were processed, resolved by SDSPAGE, transferred to nitrocellulose membranes and probed with antibodies specifi c to p53 and ▀-actin protein.