Figure 1: A549 lung cancer cells were treated with 100 �M of linoleic acid (LA), docosahexaenoic acid (DHA), or ethanol (EtOH), or 25 nM of control/nonsilencing siRNA (NS siRNA), or siRNA specific to p53 (p53 siRNA). 24 hours later siRNA treatments were supplemented with 100 �M of LA, DHA, or EtOH. 48 hours after PUFA supplementation, cells were processed, resolved by SDSPAGE, transferred to nitrocellulose membranes and probed with antibodies specifi c to p53 and �-actin protein.