Figure 2: Human lung cancer cells A549 (A) and H441 (B), or human colon cancer cells COLO 205 (C) and WiDr (D) cell lines were plated in 96-well plates and were left untreated (NT), received transfection reagent only (Mock), were treated with 25 nM control/non-silencing siRNA (NS siRNA), siRNA specifi c to p53 (p53 siRNA), or were treated with the chemical inhibitor to p53, Pifi thrin a (Pifi thrin). After 24 hours, colon cells were treated with 125 �M of linoleic acid (LA), docosahexaenoic acid (DHA), or ethanol (EtOH). Lung cells received 100 �M concentrations of the same fatty acids. Proliferation was assessed 48 hours later by MTT. Results represent at least 3 independent experiments. Statistical significance versus NT EtOH (*) and NT DHA (#, only versus other DHA treatments) groups are indicated and represent p values 0.05.