Figure 3: A549 & H441 human lung cancer cells were treated with 25 nM of control/non-silencing siRNA (NS siRNA), siRNA specifi c to p53 (p53 siRNA), or the chemical inhibitor to p53 Pifi thrin (Pifithrin). 24 hours later they were supplemented with 100µM of linoleic acid (LA), docosahexaenoic acid (DHA), or ethanol (EtOH). 48 hours after PUFA supplementation, cells were analyzed with an Annexin V FITC Apoptosis Detection Kit (Calbiochem®) by flow cytometry. The % Apoptotic/Necrotic cells were quantified from the gated population of cells in the upper and lower right quadrants. Statistical significance versus NS siRNA EtOH (*) and NS siRNA DHA (#, only versus other DHA treatments) groups are indicated and represent p values = 0.05.