Figure 3: A549 & H441 human lung cancer cells were treated with 25 nM of control/non-silencing siRNA (NS siRNA), siRNA specifi c to p53 (p53 siRNA), or the chemical inhibitor to p53 Pifi thrin (Pifithrin). 24 hours later they were supplemented with 100�M of linoleic acid (LA), docosahexaenoic acid (DHA), or ethanol (EtOH). 48 hours after PUFA supplementation, cells were analyzed with an Annexin V FITC Apoptosis Detection Kit (Calbiochem�) by flow cytometry. The % Apoptotic/Necrotic cells were quantified from the gated population of cells in the upper and lower right quadrants. Statistical significance versus NS siRNA EtOH (*) and NS siRNA DHA (#, only versus other DHA treatments) groups are indicated and represent p values = 0.05.