Figure 4: A549 lung cancer cells were treated with 25 nM of control/nonsilencing siRNA (NS siRNA), siRNA specific to p53 (p53 siRNA)and suspended in an RPMI/Soft agar solution over a basement layer. Plates were allowed to grow for 2 weeks and were rehydrated every 3 days before being stained with crystal violet before being counted (A) and photographed (B). Statistical significance versus NS siRNA EtOH (*) and NS siRNA DHA (#, only versus other DHA treatments) groups are indicated and represent p values = 0.05.