Figure 5: Protein and activity profiles of CDC25A. WISH cells were either not treated (control) or treated with 200 U of IFNγ for 24 hrs, 48 hrs or 72 hrs and the whole cell lysates (200 µg each) were: western blotted for CDC25A phosphatase (A) or assayed for CDC25A phosphatase activity (B). The densitometry values (indicated below respective lanes in the western blot) for the treated samples in the representative blot/assay have been normalised with respect to the control. The activity in the Table below is represented in terms of µmoles of pNP formed per 10 min. The fold difference in the phosphatase activity was normalised with respect to the control. Standard error for multiple independent experiments was 10%. PS: Ponceau S stained gel representing equal loading.