Figure 6: Western blot of the protein profi les of p21, p27, cyclin D and CDK4 in WISH cells treated with IFNγ. WISH cells were either not treated (control) or treated with 200 U of IFNγ for 24 hrs, 48 hrs or 72 hrs and the whole cell lysates were western blotted for: p21, p27, cyclin D and CDK4. The densitometry values (indicated below respective lanes) for the treated samples in the representative blots were normalised with respect to the control. Standard error values for multiple independent experiments were 4-8% for different proteins. PS: Ponceau S stained gel representing equal loading.