Figure 2:Characterization of azurin. (a) The Fractions collected from CM cellulose were analyzed by MALDI-TOF using Nitrogen laser at 337 nm, confirming the 14 kDa molecular weight of azurin. (b) The molecular weight was further confirmed by running the purified secreted azurin from all strains using 12% SDS-PAGE. Lane 1: standard molecular weight marker; Lane 2: azurin from P. aeruginosa MTCC 1934; Lane 3: azurin from P. aeruginosa MTCC 1942; Lane 4: azurin from P. aeruginosa MTCC 741; Lane 5: azurin from P. aeruginosa MTCC 2453.