Figure 3 : Secondary structure analysis by FTIR. Azurin from all P. aeruginosa strains were dissolved in 0.02 M PBS for analysis in Fourier Transform Infra-red (FTIR) spectroscopy. It showed azurin has C=O (protein backbone) stretching, a unique nature of the amide I band which is a characteristic feature of β-structure. FTIR spectrum also revealed there is a shift differences among azurin, synthesized by strains. Our FTIR analysis showed peak around 1646.936 in P. aeruginosa 2453 whereas, others showed peak around 1642.269, 1639.446, 1637.873 for P. aeruginosa 741, 1942, 1934 respectively.