Figure 7 :Azurin (secretory product of P. aeruginosa MTCC 2453) induced apoptosis in ZR-75-1: (a) ROS levels of ZR-75-1 treated with 72 3g/ml of azurin for 12, 24, 48 h along with control were measured after staining with 2, 7 Dichlorofluorescin diacetate (DCFH-DA) as measured by flow cytometry.. Representative histogram plots of three independent experiments. (a) Western blotting of ZR-75-1 cells treated with P. aeruginosa 2453 secreted azurin 72 3µg/ml for the indicated time in hours. (c) DNA laddering of ZR-75- 1 cells treated with azurin for the indicated time in hours.