Case

Number of Cores (Virtual/Actual)

True Value

Number Sampled

Virtual TMA

Actual TMA

Mean

95% CI

% Dec1

Mean

95% CI

% Dec1

TTF-1

3

10/8

91-95

1

96.0

89.4, 102.6

-

93.1

86.2, 100.0

-

   

 

2

96.0

91.7, 100.3

34.4

93.0

88.5, 97.6

34.2

   

 

3

96.0

92.7, 99.3

24.5

93.0

89.7, 96.3

26.7

   

 

4

96.0

93.4, 98.6

19.8

93.0

90.4, 95.6

22.3

   

 

5

96.0

93.8, 98.2

16.0

93.0

90.9, 95.0

20.6

5

10/7

81-85

1

60.2

-17.8, 138.1

-

60.6

-19.0, 140.2

-

   

 

2

59.9

5.9, 113.9

30.7

59.4

5.9, 112.9

32.8

   

 

3

60.3

20.4, 100.3

26.0

60.7

23.2, 98.1

30.0

   

 

4

59.9

28.7, 91.1

21.9

60.5

32.5, 88.6

25.2

   

 

5

60.7

34.3, 87.1

15.4

60.4

39.9, 80.9

26.9

9

9/10

1-5

1

8.4

-29.8, 46.5

-

0.9

-1.8, 3.6

-

     

2

8.3

-16.8, 33.3

34.4

0.9

-0.9, 2.7

33.5

     

3

8.5

-10.6, 27.6

23.7

0.9

-0.5, 2.3

23.3

     

4

8.4

-6.6, 23.5

21.2

0.9

-0.2, 2.0

20.3

     

5

8.4

-3.7, 20.4

20.1

0.9

0.0, 1.8

19.8

p53

3

10/10

6-10

1

5.8

-7.4, 19.0

-

3.3

-3.3, 9.8

-

   

 

2

5.7

-3.0, 14.4

34.1

3.4

-1.1, 7.9

32.0

   

 

3

5.7

-0.8, 12.3

24.6

3.4

0.0, 6.8

22.7

   

 

4

5.6

0.4, 10.8

20.6

3.4

0.7, 6.1

21.3

   

 

5

5.7

1.4, 10.0

17.0

3.4

1.2, 5.6

17.4

5

10/8

26-30

1

12.5

-1.0, 26.0

-

4.4

-6.8, 15.6

-

   

 

2

12.5

3.5, 21.6

33.3

4.5

-3.1, 12.0

32.5

   

 

3

12.5

5.6, 19.4

23.7

4.4

-1.2, 9.9

27.2

   

 

4

12.4

7.1, 17.8

22.4

4.3

0.1, 8.6

22.6

   

 

5

12.6

8.1, 17.0

16.3

4.4

1.2, 7.7

22.9

9

9/7

51-55

1

57.4

13.8, 100.9

-

61.5

10.8, 112.2

-

     

2

57.4

28.8, 86.1

34.3

61.6

28.9, 94.3

35.5

     

3

57.4

35.6, 79.2

23.9

61.5

37.5, 85.5

26.6

     

4

57.4

40.3, 74.6

21.3

61.6

43.2, 80.0

23.4

     

5

57.6

43.7, 71.5

19.0

61.6

48.5, 74.7

28.6

Ki-67

3

10/8

11-15

1

15.1

1.7, 23.4

-

5.3

-4.4, 15.1

-

   

 

2

15.0

6.1, 23.8

33.8

5.4

-1.0, 11.7

34.8

   

 

3

15.0

8.0, 21.9

21.4

5.4

0.6, 10.2

24.3

   

 

4

15.0

9.5, 20.5

21.0

5.4

1.7, 9.1

22.9

   

 

5

15.0

10.4, 19.5

16.7

5.4

2.5, 8.3

23.2

5

10/9

26-30

1

14.0

-2.9, 30.9

-

11.3

-4.0, 26.6

-

   

 

2

14.0

3.4, 24.7

36.9

11.4

1.1, 21.7

32.7

   

 

3

14.0

5.6, 22.4

21.1

11.4

3.8, 19.0

26.2

   

 

4

14.1

6.9, 21.4

13.6

11.3

5.4, 17.3

21.9

   

 

5

14.0

8.2, 19.8

20.1

11.4

6.5, 16.3

18.0

9

10/10

86-90

1

58.0

21.7, 94.4

-

52.5

10.8, 94.2

-

     

2

58.2

34.0, 82.4

33.5

52.7

25.0, 80.3

33.8

     

3

58.0

39.9, 76.1

25.2

52.2

31.1, 73.3

23.6

     

4

57.9

43.1, 72.7

18.3

52.5

35.5, 69.6

19.2

     

5

57.9

45.3, 70.5

15.0

52.5

39.0, 66.1

20.4

Table 1: Selected bootstrap resampling results for percentage of positive cells in all three antibodies (complete cores are provided in Supplementary Tables S1-S3).