Stage

Changes

Markers

Competence

LEC respond to unidentified initiating and instructive growth factor

VEGFR3 & 2 LYVE-1

Commitment/Bias

Polarization of localization LEC & subsequent budding

Prox1 LYVE-1

Specification and Coalescence

Further differentiation to achieves complete autonomy, migrates peripherally

Neuropilin 2 (Nrp2),  Podoplanin/T1 Secondary lymphoid chemokine (SLC) VEGFR3

Maturation

Organisation of primary lymphatic sac around tissues and organs

Desmoplakin and b-chemokine receptor D6