Figure 2: CaneCPI-4 does not modulate B16F10-Nex2 melanoma cell proliferation. B16F10-Nex2 melanoma cells (103) were plated in 96-well plates and incubated with CaneCPI-4 at 1 mM in complete RPMI medium. Cell viability was measured using MTT reagent. Error bars, SD of quadruplicate determinations.