Figure 3: Sugarcane cystatin CaneCPI-4 inhibited endothelial cell angiogenesis on Matrigel in vitro. HUVEC cells (5 × 103) were plated on Matrigel supplemented with 0.5% FCS in the presence of CaneCPI-4 at 0.15, 0.5 and 1 µM. The number of pro-angiogenic structures was counted after 18 h. p values in comparison with the control are shown. Error bars, SD of triplicate samples. A representative image with results of the three different treatments is shown. Scale bar = 100 µm.