Figure 7: Effect of sugarcane cystatin in the lung colonization after implantation of B16F10-Nex2 melanoma cells. C57Bl/6 mice (n=7, control group; n=5, treatedgroup) were injected intravenously with 5x105 B16F10-Nex2 melanoma cells. CaneCPI-4 (1µM, •) was administered intraperitoneally (100 µL) on days 2, 4, 6, 8, 10 and 12 after tumor cell challenge. Control mice received PBS (control, •) at the same time period. Lungs were collected 15 days after tumor challenge and the nodules were counted. (a) Total number of lung metastases. Number of lung lesions ± SE. Two independent experiments were performed. p = 0.0347, CaneCPI-4 vs control. (b) Lungs from B16-bearing mice treated with PBS and CaneCPI-4.