Figure 8: Anchorage-independent colony growth in soft agar in the presence of sugarcane cystatin. B16F10-Nex2 cells (5x103) were cultivated in soft agar in 12-well plates as described previously in the presence of CaneCPI-4 at 1 �M. After 2-3 weeks, the colonies were stained and counted. p values in comparison to the control are shown.