Figure 4: Relative telomerase activity per g protein (RTA) was measured with the tRQ-TRAP and displayed as means SEM (error bars) for each sample. The data show elevated telomerase activity in hepatocellular carcinoma (G2 and G3 HCC) and liver metastases in comparison to normal liver samples and a benign liver lesion (sample 29 I, liver adenoma). The difference in telomerase activity between cancer samples (median RTA 78.92, n = 10) and normal liver samples (median RTA 8.03, n = 4) was significant (Mann-Whitney U test, P = 0.008). The depicted line displays the cut-off value for relevant RTA-levels (above mean RTA + 2 SEM of normal liver samples).