Figure 4: Effects of FTS in combination with cytotoxic drugs or new FTS derivatives on NCIH929 cell growth. (A) NCIH929 cells (150,000 cells in 96-well plates) were plated in 5% FCS medium and then treated with FTS (50 or 75 µM) or vehicle (0.1% DMSO) combined with the indicated concentration of doxorubicin, and incubated for 5 days. Live cells were counted with the aid of the alamarBlue reagent. Inset: Shown are the numbers of cells in doxorubicin-treated samples, calculated as a percentage of the total number of cells in the control (means ± SD, n = 3). Numbers of cells in the drug-treated cultures are expressed as percentages of the numbers recorded in the zero drug (vehicle-treated) controls. Data are means of three experiments. *P < 0.05, **P < 0.001, Mann-Whitney U test. (B) NCIH929 cells (150,000 cells in 96-well plates) were plated in 5% FCS medium containing the drug at its final concentration. FTS derivates were dissolved in DMSO and diluted with 5% FCS medium to the indicated concentrations. Control cells received the vehicle (0.1% DMSO). Cells viability was determined after 4 days with the aid of the alamarBlue reagent. Data are means of three experiments.