Figure 1: Effect of different sample treatment on the monomer-oligomer state of nucleophosmin in HeLa cells (a) and comparative analysis of the monomeroligomer state of nucleophosmin in HeLa and Hep G2 cells (b). a) Samples were treated under conditions: of traditional Laemmli method at 100°C for 5 min (1), of “native” electrophoresis at 37°C for 20 min (2), of our modification at 100°C for 1 min (3); b) HeLa (1) and Hep G2 (2) cells were treated in our modification at 100°C for 1 min. SDS-PAGE was performed by the Laemmli method in 7.5% PAG. The proteins were electrotransferred in two stages. Here and in Figures 2-4 strokes show the lines of membrane section; MAb to protein B23 3C9 (a) and FC 82291 (b) were used for immunostaining; positions of marker proteins are shown on the left.