Figure 4: Western blot analysis of ceramide treated cells. Blots were analyzed against p53 monoclonal antibody. From left to right: Lane 1,2,3,4 show induction of p53 due to C6-Ceramide stimulation for 6, 12, 24 and 48 hours of incubation. Lane 5, 6, 7 and 8: Control (DMSO) without ceramide stimulation and incubated with parallel time periods 6, 12, 24, and 48 hrs of incubation.