Figure 5: Efficacy of combined ritonavir + vincristine treatment in MCL. Effects of 10 μM ritonavir treatment and/or 5 μM vincristine treatment on the proliferation of GP (panel A), GRL (panel B), and JVM2 (panel C) after 24, 48, or 72 hour incubation periods, as assessed using MTT assays. GP, GRL, and JVM2 cell lines were seeded at 10,000 cells per well in a 96 well plate and incubated with 10 μM ritonavir and/or 5 μM vincristine for 24, 48, or 72 hours. Two hours prior to the given time point, 5 mg/ml MTT was added to each well, and cells were harvested at time point using a detergentbased buffer. Proliferation of the cells was assessed calorimetrically using a spectrophotometer. * p < 0.001; # p = 0.027 comparing vincristine treatment alone to combined treatment with ritonavir and vincristine.