| DNA cloned region |
Primers |
Anneling Temperature (°C) |
Fragment (bp) |
| PCR 1 for CGI-1 |
1-F1I1
TTGATTTTAAGTTGGTTGTA |
43, 45, 47 |
684 |
| 2-R1I1
CTTTACTACAACTCTCATTCTA |
| PCR2 for CGI-1 |
3-FNI1 TTTGTTTGTTTATTTTTTATTTATTGT |
47, 49, 51 |
454 |
| 4-RNI1 ATCCCTAAACCTCTCTCAAATAAAC |
| PCR1 for CGI-2F |
5-FFI2 GGGAAGAGTTTTTTGGATTTAGAAG |
41, 43, 45 |
696 |
| 6-RFI2
TTATTATCCCTATAACAAAACC |
| PCR2 for CGI-2F |
7-FNFI2 FNGAGTTTTTAGTTTTTTGGGT |
44, 46, 48,5 |
511 |
| 8-RNFI2 CAATAAAAAACAAACAAATTAATC |
| PCR1 for CGI-2I |
9-FII2 GGATTAATTTGTTTGTTTTTTATTG |
52, 54, 56 |
711 |
| 10-RII2 AACTACCTCCACCCCCACTATAT |
| PCR2 for CGI-2I |
11-FNII2 GGGGTTTTGTTATAGGGATAATAA |
54, 56, 58 |
595 |
| PCR1 for CGI-3 |
12-FI3
GGATTTTTTAGGGATAGGGA |
44, 46, 48 |
453 |
| 13-RI3
TCTAACAAACAAACAATACTCA |
| PCR2 for CGI-3 |
14-FNI3 GTTGGGAAGGATATAGAGAGGTGGTT |
46, 48, 50 |
271 |
| 15-RNI3 AAACTAAAAACTCCCCCACCCC |
| PCR1 for CGI-5 |
16-FI5 AATGGTTTGGTTTGATGGT |
46, 48, 50 |
668 |
| 17-RI5 ACACCCACTATATACTAAACCT |
| PCR2 for CGI-5 |
18-FNI5 ATTTAGGTTGGAGTGTAG |
46, 48, 50 |
306 |
| 19-RNI5 ACAAACCTCACAATTTTA |
|