Figure 5: Association therapy decreases survival fraction and allows reducing irradiation dose. A-B. Clonogenic assay performed in 6 well plates cultured up to 15 days after XRCd10Gy, Ca2+n and association therapy condition (Ca2+n + XRCd10Gy). Representative images show a decrease of colony formation under association therapy, (***p<0.0001, t test), N = 3. C. Association therapy not only decreased the percentage of A431 cell survival fraction, but also allowed reducing irradiation by approximately half of the cumulative dose (N = 3 individual experiments).