Figure 4: SF1126 induced G1 arrest and decreased cyclin D1 in B-NHL cell lines. SUDHL-4 (A) and TMD-8 (B) cells were treated SF1126 or CAL-101 for 48 hr at varying concentrations. Samples were harvested and stained with propidium iodide. DNA content was analyzed by flow cytometry. X-axis: DNA content, Y-axis: cell numbers. Percentages of G0/G1, S and G2/M phase cell populations were analyzed by ModFit software. (C). SUDHL-4, TOLEDO and SUDHL-6 cells were treated with SF1126 for 48 hr at 5 µM, 10 µM, 20 µM, 40 µM and 50 µM. Cyclin D1 protein level was evaluated by immunoblotting.