Figure 4: A) Induction of EWS-WT1 by tetracycline in HeLaTrex cells. After induction of EWS-WT1(-KTS), the total expression levels of β-catenin were increased, together with the phosphorylated forms of Akt and MAPK. B) Transient knockdown of FGFR4 in a JN-DSRCT cell line did not alter the protein expression levels of β-catenin, the activated form of β-catenin, or cyclinD1. C) Transient knockdown of FGFR4 in a JN-DSRCT cell line did not affect significant change in cell proliferation rate.