Figure 5: PQ1 counteracts cisplatin reduction of connexins. T47D cells were treated with PQ1 and cisplatin alone and in combination as described. (A) Expression of connexin 26 (Cx26), connexin 32 (Cx32), and connexin 43 (Cx43) were examined by Western blot analysis. Actin was used as loading control. (B) Graphical presentation of three independent experiments shows levels of connexins normalized to control. Pixel intensities of protein bands were normalized to pixel intensities of actin, and the results of treated cells are normalized to the results of the controls. * P-value is <0.05 compared to control. ** P-value is <0.05 compared to cisplatin treatment.