Figure 6: Combinational treatment of PQ1 and cisplatin enhances apoptosis. T47D cells were treated with PQ1 and cisplatin alone and in combination as indicated. Cells without treatment were used as controls. (A) Expression of procaspase-9, caspase-9 p35, procaspase-8, caspase-8 p18, Bax and Bcl-2 were examined by Western blot analysis. Actin was used as loading control. (B) Graphical presentation of three independent experiments shows levels of apoptotic factors normalized to control. Pixel intensities of protein bands were normalized to pixel intensities of actin, and the results of treated cells are normalized to the results of the controls. * P-value is <0.05 compared to control. **P-value is <0.05 compared to cisplatin treatment. *** P-value is <0.05 compared to PQ1 treatment. (C) Immunofluorescence was performed to examine expression levels of cleaved caspase-3. Red is cleaved caspase-3 and blue indicates the nuclei.