Figure 2: At 7 days post treatment, tumor growth suppression is observed, but p(ser473) levels are not altered. In vitro western blot analysis of LCC6WT-luc cells illustrates that treatment with QLT0267 has a dose-dependent effect on suppression of p(ser473)AKT (A). Representative images from bioluminescent analysis of orthotopic LCC6WT-luc treated with vehicle and QLT0267 are shown (B). Total light emission from tumors in animals was visualized and quantified at the time of tumor cell inoculation (IN), and 4, 24, and 168 hours after treatment initiation. At 168 hours animals treated with QLT0267 showed lower total light flux than vehicle control (P<0.05) (C). Day 7 IHC of p(ser473)AKT was performed on a TMA containing tumorcores from vehicle and QLT0267 treated animals. Positive (breast cancer tissue) (D) and negative (primary Antibody omitted) (E) controls are shown. At this time-point, No significant difference (P>0.05) in p(ser473)AKT staining is observed when comparing tumors from vehicle (F) and QLT0267 treated (G) animals as quantified by positive pixel counting (H).