Figure 7: TWIST expression is suppressed by QLT0267 in vivo. Vehicle and QLT0267 treated tumor cores were subject to TWIST IHC. Subsequently, quantification of staining was performed using the Aperio Image Scope software. Tonsil tissue was used as the negative control (A). At 24 hours, Vehicle treated tumor samples showed cytoplasmic, peripheral and nuclear staining of TWIST. Comparatively, QLT0267 treated tumors from the same time-point exhibit an overall decrease in TWIST with little to no evidence of peripheral and nuclear staining (B).Quantification of TWIST expression showed that TWIST is significantly decreased in QLT0267 treated tumors as compared to vehicle controls at 24 hours (P<0.05) (C).