Figure 1: SDS-PAGE gel showing the purification of ADAM33–HisTag. Soluble extract of ADAM33 recombinant protein expressed in E. coli. BL21Ai was purified in a Hi-Trap Ni-Chelating column (GE Healthcare). Some proteins cannot be detected in the flow through fraction (lane 1-4). Recombinant protein was eluted using an imidazole gradient (from 20 mM to 1M) (lanes 5-10).Samples were separated by SDS–PAGE and stained with Coomassie blue.