Figure 2: Validation of apoptosis and cell cycle arrest related genes (A, B, C) and proteins(D, E, F) in salinomycin-treated PC-3 cells. (A, D) Cyclin expression, (B, E) Cyclin-dependent kinase expression, (C, F) Cyclindependent kinase inhibitors expression. Semiquantitative RT-PCR analysis for genes expression and Western blot analysis for proteins expression were performed by using PC-3 cells treated with 1.33 μM salinomycin for various times. GAPDH and β-actin were used as an internal control, respectively.