Figure 3: Validation of apoptosis and ER stress/UPR related genes (A, B) and proteins(C, D) in salinomycin-treated PC-3 cells. (A, C) ER stress sensors expression. (B, D) ER stress related transcript factors expression. Semiquantitative RT-PCR analysis for genes expression and Western blot analysis for proteins expression were performed by using PC-3 cells treated with 1.33 μM salinomycin for various times. GAPDH and β-actin were used as an internal control, respectively.