Primer sequence of RIZ-1 Primer AT Product size
5’GTGGTGGTTATTGGGCGACGGC-3’ 5’-GCTATTTCGCC­GACCCCGACG-3’ 5’-TGGTGGTTATTGGGTGATGGT-3’ 5’-ACTATTTCACCAACCCCAAGA-3’ Methylated primer Reverse primer Unmethylated primer Reverse primer 68°C

60°C
177 bp

175 bp
Table 3: Sequence of oligonucleotides used for methylated and unmethylated DNA.