Primer sequence of RIZ-1
Primer
AT
Product size
5’GTGGTGGTTATTGGGCGACGGC-3’ 5’-GCTATTTCGCCGACCCCGACG-3’ 5’-TGGTGGTTATTGGGTGATGGT-3’ 5’-ACTATTTCACCAACCCCAAGA-3’
Methylated primer Reverse primer Unmethylated primer Reverse primer
68°C 60°C
177 bp 175 bp
Table 3:
Sequence of oligonucleotides used for methylated and unmethylated DNA.