Figure 2a: SETD4 is over expressed in ER-negative breast cancer. SETD4 mRNA expression was evaluated by qPCR. The SETD4 expression values for the HCC-1954, MCF-7, CAMA-1, SKBR-3, MDA-MB 231, MDA-MB 436 and MDA-MB 468 cells were normalized to that of the HCC-1954-BL control cells.