Figure 3a: SETD4 is localized in both the cytoplasm and nucleus. MDA-MB 231 cells were grown on glass slides prior to fixation and immunofluorescence microscopy using an antibody specific for the SETD4 protein and a secondary antibody conjugated to Alexa Fluor 488 (green). Nuclear staining with Hoechst is shown in blue. Serial optical sections were collected for three-dimensional reconstruction; x-z sections are shown at the top and y-z sections are shown on the right. Scale bar=10 μm.