Cell line Fold change p-value
MACL-1 9.5 4.01×10-6
     
MGSO-3 3.43 0.025
Table 2: Gene expression level (fold change) of SETD4 in the MACL-1 and MGSO-3 cell lines compared to normal cells (non-cancerous), as assessed by cDNA microarray. RNA samples from MACL-1, MGSO-3 and normal cells were extracted using RNeasy Mini Kit (Qiagen, Venlo, The Netherlands), following manufacturer’s protocol and cleaned from DNAse with RQ1 RNase-free DNAse (Promega). Double-strand cDNA was synthesized using SuperScript Double-Stranded cDNA Synthesis Kit (Life Technologies), according to manufacturer’s directions and cDNA quality was then evaluated on BioAnalyzer 2100 (Agilent Technologies, Palo Alto, CA, USA). Human Gene Expression 12x135K array chip (Roche NimbleGen Inc., Madison WI, USA) was used to assess gene expression profile with each cell line represented in triplicate. After Cy3 labeling, the cDNA was hybridized to the chip and slides were scanned at 532nm. Internal fluorescence was adjusted and sample tracking control was verified to exclude cross contamination between samples.