Enzyme purification step |
DNA pol β–specific catalytic activity, [3H]DNA c.p.m./mg protein
(M ± SEM, n=6) |
Total cell homogenate |
588 ± 63 |
Crude nuclei (P0.8) |
1,864 ± 87 |
Crude chromatin |
2,476 ± 118 |
Phenol-chloroform extraction |
6,081 ± 202 |
Ammonium sulfate 30%-70%
saturation gradient |
10,112 ± 233 |
Diaflo Y5.0 ultrafiltration |
19,423 ± 270 |
TOYOPEARL HW 55F
Gel Chromatography |
32,687 ± 331 |
|