Figure 1: Identification of nimesulide as inhibitor of activated Wnt/β-catenin signaling and gastric cancer cell proliferation. A) Validation of the authenticity of AGS-Wnt reporter cell line through genetic and chemical modulations of intrinsic Wnt/β-catenin pathway activity. B) Screening of a compound library in AGS-Wnt reporter stable cell line identifies Wnt inhibitory effect of Nimesulide (circled one also confirmed by secondary screen). C)Transient TCF reporter assay showing the inhibition of Wnt activity by nimesulide in a dose dependent manner in AGS cells. D) Dose dependent inhibition of cell viability by nimesulide in AGS cells at 48 hours of treatment (MTT assay). E) Proliferation pattern of gastric cancer cell lines upon nimesulide (100 μM) treatment measured by MTT assay (***P = < 0.001, ****P = < 0.0001).