Figure 3: Nimesulide inhibits Wnt/β-catenin signaling through COX-2 independent mechanism. A) Comparative Wnt signaling activity and PTGS2 (COX-2) expression across gastric cell lines as measured by reporter assay and RT-PCR respectively. B) Reporter assays indicating the positive regulation of Wnt/β-catenin and CREB to PGE2 treatment in AGS cells. C) Dose dependent inhibition of TCF activity by nimesulide in the presence of PGE2 in AGS cells. D) Wnt reporter activity in Kato-III cells upon the treatment of PGE2. E) Inhibition of TCF reporter activity by nimesulide in a dose dependent manner in Kato-III cells (COX-2 negative cell line) indicate COX-2 independency (*P = < 0.05, ***P = < 0.001).