Figure 4: Nimesulide alters the activity of STAT3, RXR and IRF1 signaling in gastric cancer cells. A)Comprehensive screening of nimesulide against 45 signaling pathways by corresponding transcription factor activity reporter assay in AGS cells. B-D) Nimesulide mediated inhibition of Wnt, IRF1, RXR and STAT3 pathways in AGS (B), MKN45 (C) and YCC3 (D) cell lines evaluated by reporter assay. E) RT-PCR showing nimesulide mediated downregulation of the expression of IF127, IFI44, and NEDD9, the target genes of IRF1 and RXR signaling pathways in AGS cells.