Figure 5: Nimesulide regulates RXR, IRF1 and STAT3 response through COX-2 dependent/independent mechanism. A) Analysis of RXR, IRF1 and STAT3 response upon 12 hours treatment of PGE2 by reporter assay. B) STAT3 pathway is inhibited by nimesulide even in the presence of PGE2 and indicating COX2 independent mode of inhibition. C) Evaluation of Wnt mediated regulation of c-Myc, E2F1, IRF1, RXR and STAT3 activity upon CTNNB1 knockdown followed by reporter assay in AGS cell line. RT-PCR evaluation of CTNNB1 knockdown is shown inside.