Table A Hematologic responses |
Complete or major hematological response |
Partial or minor hematological response |
Loos or minimal hematological response |
Platelet count >150x 109/L
WBC count < 10 x 109/L
Basophils :< 5%
Differential without immature granulocytes.
Absence of blasts and promyelocytes in peripheral blood
Spleen : nonpalpable spleen |
Platelet count <450 x 109/L
WBC count >10 x 109/L
Basophils :>10%
Presence of blasts and promyelocytes in peripheral blood
Spleen : Palpable spleen |
Platelet count < 450 x 109/L
WBC count >20 x 109/L
Basophils :15%
Presence of blasts and promyelocytes in peripheral blood
Spleen : Palpable spleen |
Table B Molecular response |
Major molecular response |
Minimal or No Molecular response |
It indicates nonquantifiable and nondetectable bcr-abl gene transcript (BCR-ABL/ABL) 0.103
3 log reduction of BCR-ABL/ABL |
It indicates quantifiable and detectable bcr-abl gene transcript (BCR-ABL/ABL) ≥0.103
No 3 log reduction of BCR-ABL/ABL |
C5e |
5’ATAGGATCCTTTGCAACCGGGTCTGAA3’ |
B2B |
5’ACAGAATTCCGCTGACCATCAATAAG3’ |
BCR-C |
5’ ACCGCATGTTCCGGGACAAAAG3’ |
CA3 |
5’TGTTGACTGGCGTGATGTAGTTGCTTGG3’ |
Transcript |
primers used |
Size (bp) |
Control BCR |
B2B and C5e |
808bp |
b2a2 |
B2B and CA3 |
310bp |
b3a2 |
B2B and CA3 |
385bp |
e1a2 |
BCR-C and CA3 |
481bp |