Name Primer sequence AT
Unmethylated forward primer GGAGGATAGTTGGATTGAGTTAATGTT 60°C
Unmethylated Reverse primer CAAATCCCTCCCAAACACCAA
Methylated forward primer GGATAGTCGGATCGAGTTAACGTC 60°C
Methylated Reverse primer CCCTCCCAAACGCCGA
Table2: Sequence of oligonucleotides used for methylated and unmethylated DNA.