Figure 5: Adiponectin-induced SW480 cell migration via an autocrine IL-8 and MCP-1 release. A, expression of the interleukin-8 receptors CXCR1 and CXCR2 on the surface of SW480 cells was determined using flow cytometry. The antibody-bound FITC-fluorescence of specific antibodies (black line) was compared to an unspecific isotype control (gray area). MFI = mean fluorescence intensity of specific (CXCR1 and CXCR2) and isotypic (Iso) binding. The shown histogram holds true for 3 measurements that have been performed independently. B, the adiponectin-induced migratory activity of SW480 cells was inhibited by additional treatment of the cells with 10 μg/ml of an IL-8-neutralizing antibody. C, incubation of SW480 cells with 10 μg/ml MCP-1-neutralizing antibodies abolished the adiponectin-mediated cell locomotion. D, stimulation of SW480 cell migration with 100 ng/ml IL-8 and 20 ng/ml MCP-1 alone or in combination. The graphs show mean values of 3 independent experiments (90 cells were analyzed per sample).