Target gene Ct value [control] Ct value [treated] ▲Ct value [control] ▲Ct value [treated] ▲▲Ct value 2-∆∆Ct value Gene expression HPRT [reference] 31.465 30.967 - - 0 1 ____ BIRC 3 31.482 29.084 0.017 0.883 0.866 0.548 1.8 fold decrease BIRC 4 31.514 31.213 0.049 0.246 0.197 0.872 1.2 fold decrease DR4 29.286 28.408 -2.179 -2.559 -0.38 1.301 1.3 fold increase DR5 33.52 31.695 2.055 0.728 -1.327 2.508 2.5 fold increase DCR 1 34.432 32.643 2.967 1.676 -1.291 2.446 2.5 fold increase DCR 2 31.274 29.195 -0.191 -1.772 -1.581 2.99 ~3 fold increase c-FLIP 29.363 28.632 -2.102 -2.335 -0.233 1.175 ~1.2 fold increase

Table 9: Effect on 1nM leptomycin on the change in gene expression of a specific set of genes related to apoptosis in PC3 cells using Ct values obtained by RT-PCR.