Target gene Ct value [control] Ct value [treated] ▲Ct value [control] ▲Ct value [treated] ▲▲Ct value 2-∆∆Ct value Gene expression HPRT [reference] 31.209 31.479 0 0 0 1 ____ BIRC 3 31.823 30.282 0.614 -1.197 -1.811 3.508 3.5 fold increase BIRC 4 31.012 29.472 -0.197 -2.007 -1.81 3.506 3.5 fold increase DR4 29.292 30.012 -1.917 -1.467 0.45 0.732 1.36 fold decrease DR5 33.202 31.014 1.993 -0.465 -2.458 5.494 ~5.5 fold decrease DCR 1 34.629 30.362 3.42 -1.117 -4.537 23.215 23 fold increase DCR 2 31.013 28.254 -0.196 -3.225 -3.029 8.162 8 fold increase c-FLIP 29.328 26.029 -1.881 -5.45 -3.569 11.867 ~12 fold increase

Table 10: Effect on 5nM leptomycin on the change in gene expression of a specific set of genes related to apoptosis in PC3 cells using Ct values obtained by RT-PCR.