Target gene Ct value [control] Ct value [treated] ▲Ct value [control] ▲Ct value [treated] ▲▲Ct value 2-∆∆Ct value Gene expression HPRT [reference] 32.232 32.38 0 0 0 1      ____ BIRC 3 31.123 30.853 -1.109 -1.527 -0.418 1.336 1.3 fold increase BIRC 4 31.732 31.388 -0.5 -0.992 -0.492 1.406 1.4 fold increase DR4 32.32 33.53 0.088 1.15 1.062 0.478 ~2 fold decrease DR5 31.532 30.292 -0.7 -2.088 -1.388 2.617 2.6 fold increase DCR 1 32.824 31.568 0.592 -0.812 -1.404 2.646 2.65 fold increase DCR 2 31.721 30.856 -0.511 -1.524 -1.013 2.018 2 fold increase c-FLIP 30.327 29.509 -1.905 -2.871 -0.966 1.953 ~2 fold increase

Table 13: Effect of 1nM leptomycin B on the change in gene expression of a specific set of genes related to apoptosis in MCF 7 cells using Ct values obtained by RT-PCR.