Figure 4: Boldine inhibits glioma growth in vivo. Animals were treated, as described in Section 2. Tumor size was measured 20 days after implantation of C6 cells by three hematoxylin and eosin (H&E) sections of each tumor. For tumor size quantification, images were captured using a digital camera connected to a microscope and total volume (mm3) was determined using Image Tool SoftwareTM. (A) Representative tumor images of the control, vehicle and boldine-treated groups, magnification 10x; (B) Tumor size quantification of implanted gliomas. The values are represented as means ± S.D (Control group – 6 animals; Vehicle group – 6 animals; boldine-treated group – 8 animals). Data were analyzed by ANOVA followed by Tukey’s test. *** p < 0.001.