Figure 1: Effects of RP215, anti-human IgG and anti-T cell receptors on induced apoptosis of cancer cells. A) Induced apoptosis on treated cultured OC-3- VGH cancer cells by normal mouse IgG (NMIgG) (Lane 1), normal rabbit IgG (NRIgG) (Lane 2), mRP215 (Lane 3), hRP215 (Lane 4), goat anti-human IgG (GαHIgG) (Lane 5), rabbit anti-T cell receptors β (RαTCRβ) (Lane 6) and anti-TLR4 (αTLR4) (Lane 7) for 24h incubation. □ And ■ represent 1 μg/ml and 10 μg/ml, respectively of the ligands used for the apoptosis assay. B)  Represents the corresponding data obtained for 48h incubation (10 μg/ml). C) Induced apoptosis of several cultured cancer cells including DU-145 (prostate) (Lane 1), A549 (lung) (Lane 2), C-33A (cervix) (Lane 3) and MDA-MB-435 (breast) (Lane 4) [6,9]. , and represent the treatments with normal mouse IgG (NMIgG), mRP215 and goat anti-human IgG (GαHIgG) (10 μg/ml), respectively. D) Complement-dependent cytotoxicity (CDC) reactions in the presence of 10 μg/ml each of mRP215, hRP215 and antibodies against antigen receptors, as well as normal immunoglobulins used as the negative control. Lane 1: no treatment (no treat.); Lane 2: 3 μL freshly prepared rabbit baby complement (comp. only); Lane 3: normal mouse IgG plus complement (NMIgG); Lane 4: normal human IgG plus complement (NHIgG); Lane 5: normal rabbit IgG plus complement (NRIgG); Lane 6: mRP215 plus complement (mRP215); Lane 7: hRP215 plus complement (hRP215); Lane 8: goat anti-human IgG plus complement (GαHIgG); Lane 9: rabbit anti-T cell receptors β plus complement (RαTCRβ); Lane 10: anti-TLR4 plus complement (αTLR4). All data presented are statistically significant at * P<0.05, ** P<0.01 and *** P<0.001.